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Isolation of Leukocytes from the Human Maternal-fetal Interface
1Perinatology Research Branch,NICHD/NIH/DHHS, 2Department of Obstetrics and Gynecology,University of Michigan, 3Department of Epidemiology and Biostatistics,Michigan State University, 4Department of Molecular Obstetrics and Genetics,Wayne State University, 5Department of Obstetrics and Gynecology,Wayne State University School of Medicine, 6Department of Immunology and Microbiology,Wayne State University School of Medicine
Chapters
- 00:05Title
- 01:41Dissection of Human Decidual Tissues
- 03:36Mechanical Disaggregation and Enzymatic Digestion
- 04:31Isolation of Leukocytes
- 06:22Results: Leukocyte Isolation from Maternal-fetal Interface
- 07:46Conclusion
Described herein is a protocol to isolate and further study the infiltrating leukocytes of the decidua basalis and decidua parietalis - the human maternal-fetal interface. This protocol maintains the integrity of cell surface markers and yields enough viable cells for downstream applications as proven by flow cytometry analysis.
