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Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain
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神经科学
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JoVE 杂志 神经科学
Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain

Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain

DOI:
10.3791/52853-v

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06:58 min

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June 01, 2015

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, , , ,
1Department of Pharmacology and Experimental Therapeutics,University of Toledo, College of Pharmacy and Pharmaceutical Sciences, 2Life Sciences Institute,University of Michigan, 3Department of Biomedical & Pharmaceutical Sciences,Chapman University, School of Pharmacy, Rinker Health Science campus

Chapters

  • 00:05Title
  • 01:31Brain Extraction, Sectioning, and Tissue Preparation
  • 02:28Live Imaging Configuration and Setup
  • 04:00Calcium Signal Recording and Immunofluorescence Microscopy
  • 05:26Results: Measuring Ependymal Cilia Function and Calcium Signaling
  • 06:22Conclusion

Summary

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Using high-resolution differential interference contrast (DIC) microscopy, an ex vivo observation of the beating of motile ependymal cilia located within the mouse brain ventricles is demonstrated by live-imaging. The technique allows a recording of the unique ciliary beating frequency and beating angle as well as their intracellular calcium oscillation pacing properties.

Tags

Live ImagingEpendymal CiliaLateral VentriclesMouse BrainMulticiliated Ependymal CellsCiliary DynamicsCiliary Beating FrequencyCalcium OscillationsImmunofluorescence ImagingNeurological Deficits

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