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Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons
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JoVE Journal Neuroscience
Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons

Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons

DOI:
10.3791/52846-v

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10:57 min

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July 13, 2015

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, ,
1Translational Neuroscience Facility, School of Medical Sciences,University of New South Wales

Chapters

  • 00:05Title
  • 01:58Acetylcholinesterase Histochemical Staining
  • 02:49Intramuscular Injections at the Motor End Plates
  • 04:49Cervical Spinal Cord Dissection and Histology Preparation
  • 07:35Lumbar Spinal Cord Dissection and Histology Preparation
  • 08:49Results: Representative Motor End Plate Staining and Analysis
  • 10:16Conclusion

Summary

Automatic Translation

Automatic Translation

The efficacy of intramuscular uptake and retrograde transport of molecules to corresponding motor neurons depends on the location of the injection sites with respect to the motor end plates (MEPs). Here, we describe how to locate MEPs on skeletal muscles to optimise retrograde transport of tracers into motor neurons.

Tags

Keywords: Intramuscular InjectionsMotor End PlatesRetrograde TransportMotor NeuronsNeuromuscular DisordersGene DeliverySkeletal MusclesSpinal CordMinimally Invasive ApproachRetrograde Tracers

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