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Chapters
- 00:05Title
- 01:32Cell Encapsulation into Alginate Hydrogel Capsules and Treatment of Alginate Capsules
- 03:44Culture and Collect Cells in Alginate Capsules
- 04:56Results: Visualizations of Alginate Capsule Formation and Morphologies of Mouse iPS Cells in Capsules
- 06:43Conclusion
We established a method of encapsulating pluripotent stem cells (PS cells) into alginate hydrogel capsules using a co-axial nozzle. This prevents cells from aggregating excessively and limits the shear stress experienced by cells in suspension culture. The technique is applicable to the mass production of PS cells as well as research on stem cell niche.
