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Bir Koaksiyel Memesini kullanma Pluripotent Kök Hücre Aljinat Encapsulation
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发育生物学
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JoVE 杂志 发育生物学
Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
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Bir Koaksiyel Memesini kullanma Pluripotent Kök Hücre Aljinat Encapsulation

12,373 Views

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07:13 min

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July 02, 2015

DOI:

10.3791/52835-v

DOI:
10.3791/52835-v

•

07:13 min
July 02, 2015

•

15 Views
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1Center for International Research on Integrative Biomedical System, Institute of Industrial Science,The University of Tokyo

Summary

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We established a method of encapsulating pluripotent stem cells (PS cells) into alginate hydrogel capsules using a co-axial nozzle. This prevents cells from aggregating excessively and limits the shear stress experienced by cells in suspension culture. The technique is applicable to the mass production of PS cells as well as research on stem cell niche.

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Alginate EncapsulationPluripotent Stem CellsCo-axial NozzleSuspension CultureAggregationShear StressCapsule SizeAlg-Ca CapsuleAlg-PLL CoatingStem Cell NicheMass Production

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Cite this Article

Horiguchi, I., Sakai, Y. Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle. J. Vis. Exp. (101), e52835, doi:10.3791/52835 (2015).

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