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Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
Chapters
- 00:05Title
- 02:21Punch Biopsy
- 06:12Infection of Human Fibroblasts with Sendai Virus and Transdifferentiation
- 08:47Isolation of Putative Induced Neural Progenitor Cells (iNPC) Colonies
- 10:15Differentiation of iNPCs
- 12:04Results: Reprogramming of Fibroblasts into Neural Precursors and their Cellular Validation
- 13:20Conclusion
Generation of induced pluripotent stem cells provides fascinating prospects for the derivation of autologous transplants. However, progression through a pluripotent state and laborious re-differentiation still hinders clinical translation. Here we describe the derivation of adult human fibroblasts and their direct conversion into induced neural progenitor cells and the subsequent differentiation into neural lineages.
