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Een nieuwe toepassing van de elektrische Penetration Graph (EPG) voor het verwerven en het meten van elektrische signalen in Floëem Sieve Elements
JoVE 杂志
环境科学
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JoVE 杂志 环境科学
A New Application of the Electrical Penetration Graph (EPG) for Acquiring and Measuring Electrical Signals in Phloem Sieve Elements

Een nieuwe toepassing van de elektrische Penetration Graph (EPG) voor het verwerven en het meten van elektrische signalen in Floëem Sieve Elements

DOI:

14:16 min

July 02, 2015

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Chapters

  • 00:05Title
  • 03:18Aphid Rearing
  • 04:14Making an Insect Electrode
  • 05:58Wiring an Insect for Electrical Penetration Graph (EPG) Recordings
  • 08:27EPG Recording
  • 11:27Results: Electrical Response to Wounding in Central and Marginal Veins in Leaves of Arabidopsis thaliana
  • 13:18Conclusion

Summary

自动翻译

Electrical Penetration Graph (EPG) is a well-established technique for studying the feeding behavior of stylet-bearing insects. Here we show a new application of EPG as a non-invasive tool for the acquisition of intracellular electrophysiology recordings of sieve elements (SEs), the cells that form the phloem vasculature in plants.

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