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Chapters
- 00:05Title
- 02:00Automated Cell Enrichment
- 05:03Cell Count Determination
- 06:35Examination of the Separation Performance
- 07:54Results: Enrichment of CMV-specific T Cells Using the CCS System
- 09:29Conclusion
The goal of this protocol is to manufacture pathogen-specific clinical-grade T cells using a bench-top, automated, second generation cell enrichment device that incorporates a closed cytokine capture system and does not require dedicated staff or use of a GMP facility. The cytomegalovirus pp65-specific-T cells generated can be directly administered to patients.
Tags
Automated Cell EnrichmentCytomegalovirus-specific T CellsClinical ApplicationsCytokine-capture SystemAllogeneic Hematopoietic Stem-Cell TransplantationViral-specific T CellsGamma-interferonCytokine Capture System (CCS)CliniMACS ProdigyMagnetic Activated Cell SortingCMV Pp65-specific T CellsAutomated Cell Enrichment DeviceGMP FacilitiesBlood Banking Facilities
