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Karakterisering av Thymus- Bosetting stamfedre i Mouse Embryo hjelp<em> I Vivo</em> Og<em> In Vitro</em> Analyser
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
DOI:

08:56 min

June 09, 2015

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Chapters

  • 00:05Title
  • 01:51Embryo Dissection
  • 02:52Thymus Isolation
  • 03:49E14 Thymic Lobe Progenitor Colonization: Hanging Drop Technique
  • 04:53Grafting Under the Kidney Capsule
  • 06:22Results: Representative Thymic Settling Progenitor (TSP) Propagation
  • 08:07Conclusion

Summary

自动翻译

This article describes in vivo and in vitro methodology to characterize the thymic settling progenitors by the analysis of the kinetics of generation, phenotype and numbers of their T cell progeny.

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