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पित्त चालित जिगर पुनर्जनन अध्ययन करने के लिए zebrafish में Hepatocyte-विशिष्ट एबलेशन
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
DOI:

08:14 min

May 20, 2015

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Chapters

  • 00:05Title
  • 01:30Embryo/larva Preparation
  • 02:42Metronidazole (Mtz) Treatment and Biliary-driven Liver Regeneration Analysis
  • 06:07Results: Representative Liver Ablation and Regeneration Analysis
  • 07:24Conclusion

Summary

自动翻译

To help assess the molecular mechanisms underlying zebrafish biliary-driven liver regeneration, we established a liver injury model in which the nitroreductase-expressing hepatocytes are genetically ablated upon metronidazole treatment. In this protocol, we describe how to adeptly manipulate, monitor and analyze hepatocyte ablation and biliary-driven liver regeneration.

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