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Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
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Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

DOI:
10.3791/52749-v

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08:21 min

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April 29, 2015

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, ,
1Centre de recherche du Centre hospitalier de l’Université de Montréal,Institut du cancer de Montréal, 2Département de Médecine,Université de Montréal

Chapters

  • 00:05Title
  • 01:39Isolation, Identification and Culture of Murine Peritoneal Macrophages
  • 03:30Cell Treatments
  • 04:21RNA Isolation
  • 06:57Results: TLR2 and TLR4 Ligands Induce Hepcidin Expression in Murine Peritoneal Macrophages
  • 07:55Conclusion

Summary

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We describe here a simple protocol to isolate murine peritoneal macrophages. This procedure is followed by RNA extraction to carry out gene expression analysis upon Toll-like receptors stimulation.

Tags

Peritoneal MacrophagesToll-like ReceptorsGene ExpressionInflammationMonocyte MigrationBrewer’s Thioglycollate MediumTLR LigandsImmunological StudiesMetabolic Studies

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