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High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Chapters
- 00:05Title
- 01:18Preparation of Standard Serial Dilutions, Primer/Probe, and No Template Control Working Stock Mixes
- 03:18Preparation of 384-well Assay Plates
- 05:47Loading and Running the Assay Plate
- 08:13Results: Analysis of Quantitative RT-PCR IFN Data
- 09:29Conclusion
This protocol describes a high-throughput qRT-PCR assay for the analysis of type I and III IFN expression signatures. The assay discriminates single base pair differences between the highly similar transcripts of these genes. Through batch assembly and robotic pipetting, the assays are consistent and reproducible.
