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EPA שיטת המדידה של 1615. Enterovirus ומופע norovirus במים על ידי תרבות וRT-qPCR. חלק שלישי. איתור וירוסים על ידי RT-qPCR
JoVE 杂志
环境科学
This content is Free Access.
JoVE 杂志 环境科学
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
DOI:

12:32 min

January 16, 2016

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Chapters

  • 00:05Title
  • 02:03Tertiary Concentration and Nucleic Acid Isolation
  • 03:21Reverse Transcription
  • 05:57Real Time Quantitative PCR
  • 09:23Results: RT-qPCR Determination of Enterovirus and Norovirus in Water
  • 11:38Conclusion

Summary

自动翻译

Here we present a procedure to quantify enterovirus and norovirus in environmental and drinking waters using reverse transcription-quantitative PCR. Mean virus recovery from groundwater with this standardized procedure from EPA Method 1615 was 20% for poliovirus and 30% for murine norovirus.

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