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A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

DOI:
10.3791/52640-v

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08:35 min

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March 17, 2015

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, , ,
1MSTP, Neuroscience Program,Washington University in St. Louis, 2Washington University Pain Center, Department of Anesthesiology,Washington University in St. Louis

Chapters

  • 00:05Title
  • 01:14Preparing the Testing Plate and Enclosures
  • 02:03Performing the Cold Plantar Assay (CPA) at Fixed Temperatures
  • 05:10Testing the Mice During Cold Adaptation
  • 05:54Results: Measuring Analgesia and Hypersensitivity Using the Cold Planar Assay
  • 08:00Conclusion

Summary

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自动翻译

The Cold Plantar Assay (CPA) measures cold responsiveness between 30 °C and 5 °C, and can also measure cold adaptation. This protocol describes how to use the CPA to measure cold hypersensitivity, analgesia, and adaptation in mice.

Tags

Keywords: Cold SensitivityCold HypersensitivityCold Plantar AssayCold AdaptationThermal ResponseCold AnalgesiaCold NociceptionMouse BehaviorTemperature Sensitivity

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