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Protocoles pour l'obtention Zygotic et embryons somatiques pour l'étude de la réglementation de développement précoce de l'embryon dans la légumineuse modèle<em> Medicago truncatula</em
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
DOI:

07:32 min

June 09, 2015

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Chapters

  • 00:05Title
  • 01:22Zygotic Embryo Development
  • 02:52Somatic Embryo Development In Vitro
  • 05:41Results: Representative Analyses of Somatic and Zygotic Embryo Development
  • 06:45Conclusion

Summary

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The goal is to illustrate that the model legume Medicago truncatula can be readily utilized to investigate the regulation of early plant embryogenesis to complement the non-legume Arabidopsis model. Pod morphology is linked to zygotic embryogenesis stages and a protocol to collect embryos using tissue culture is also provided.

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