Journal
/
/
Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons

Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons

DOI:

09:43 min

May 25, 2015

, , ,

Chapters

  • 00:05Title
  • 01:40Preparations
  • 02:43Compound Muscle Action Potential (CMAP) Recording
  • 03:28Isolating and Staining the Diaphram
  • 06:06Imaging and NMJ Analysis
  • 08:00Abnormal Peak CMAP Measurements and NMJ Morphologies
  • 09:17Conclusion

Summary

自动翻译

Compound muscle action potential recording quantitatively assesses functional diaphragm innervation by phrenic motor neurons. Whole-mount diaphragm immunohistochemistry assesses morphological innervation at individual neuromuscular junctions. The goal of this protocol is to demonstrate how these two powerful methodologies can be used in various rodent models of spinal cord disease.

Related Videos

Read Article