Journal
/
免疫与感染
/
Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish

Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish

DOI:
10.3791/52597-v

•

09:39 min

•

December 26, 2014

•

, , ,
1Molecular Pathology, Cancer Center and Center for Regenerative Medicine,Massachusetts General Hospital, 2Harvard Stem Cell Institute, 3GABBA – Instituto de Ciências Biomédicas Abel Salazar,Universidade do Porto

Chapters

  • 00:05Title
  • 02:01Preparation of Adult Zebrafish Donor Skeletal Muscle Cells
  • 03:57Intramuscular Transplantation of Skeletal Muscle Cells into Adult Zebrafish
  • 05:02Embryonal Rhabdomyosarcoma (ERMS) Tumor Preparation and Transplantation
  • 07:04Results: Skeletal Muscle Engraftment
  • 08:39Conclusion

Summary

自动翻译

自动翻译

Here, we present a protocol for cell transplantation of zebrafish skeletal muscle and embryonal rhabdomyosarcoma (ERMS) into adult immune compromised rag2E450fs homozygous mutant zebrafish. This protocol allows for the efficient analysis of regeneration and malignant transformation of muscle cells.

Tags

ZebrafishCell TransplantationSkeletal MuscleRhabdomyosarcomaRag2 MutantImmune CompromisedEngraftmentRegenerationCancer

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Flow Cytometry Analysis of Immune Cells Within Murine Aortas

Isolation of Functional Cardiac Immune Cells

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish

In vivo Imaging Method to Distinguish Acute and Chronic Inflammation

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds

Read Article