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Automated quantification des cellules hématopoïétiques - Interactions cellulaires stromales dans les images histologiques de décalcifiées os
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发育生物学
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JoVE 杂志 发育生物学
Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone
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Automated quantification des cellules hématopoïétiques - Interactions cellulaires stromales dans les images histologiques de décalcifiées os

11,373 Views

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09:31 min

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April 08, 2015

DOI:

10.3791/52544-v

DOI:
10.3791/52544-v

•

09:31 min
April 08, 2015

•

1 Views
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1Immunodynamics,German Rheumatism Research Center, a Leibniz Institute, 2Biophysical Analytics,German Rheumatism Research Center, a Leibniz Institute, 3Max-Delbrück Center for Molecular Medicine, 4Wimasis GmbH, 5Immunodynamics and Intravital Imaging,Charité – University of Medicine

Summary

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A strategy to quantitatively analyze histological data in the bone marrow is presented. Confocal microscopy of fluorescently labeled cells in tissue sections results in 2-dimensional images, which are automatically analyzed. Co-localization analyses of different cell types are compared to data from simulated images, giving quantitative information about cellular interactions.

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Automated QuantificationCell-cell InteractionsHematopoietic CellsStromal CellsBone MarrowConfocal MicroscopyHistological AnalysisCell RecognitionNeighborhood AnalysisCo-localizationRandom Positioning

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Cite this Article

Zehentmeier, S., Cseresnyes, Z., Escribano Navarro, J., Niesner, R. A., Hauser, A. E. Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone. J. Vis. Exp. (98), e52544, doi:10.3791/52544 (2015).

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