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1Center for Gastrointestinal Biology and Disease,University of North Carolina at Chapel Hill, 2Department of Microbiology and Immunology,University of North Carolina at Chapel Hill, 3Department of Genetics,University of North Carolina at Chapel Hill, 4Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill
Chapters
- 00:05Title
- 01:18Isolation of Splenic T Cells from Donor Mice
- 02:41Enrichment of CD4+ T Cells
- 04:34Labeling and Sorting the CD4+ T Cells
- 06:31Injection of Cells into Recipient Mice and Monitoring of Disease Progression
- 07:22Results: Colitis Progression After Adoptive T Cell Transfer into Recipient Mice
- 08:15Conclusion
Here, we present a protocol to induce colonic inflammation in mice by adoptive transfer of syngeneic CD4+CD45RBhigh T cells into T and B cell deficient recipients. Clinical and histopathological features mimic human inflammatory bowel diseases. This method allows the study of the initiation of colonic inflammation and progression of disease.
