This content is Free Access.
Chapters
- 00:05Title
- 01:39Solid-state Hydrogen Deuterium Exchange Mass Spectrometry for Intact Protein
- 04:34Solid-state Hydrogen Deuterium Exchange Mass Spectrometry for Proteins at the Peptide Level
- 06:23Solid-state Photolytic Labeling for Intact Protein
- 07:36Solid-state Photolytic Labeling for Poteins at the Peptide Level
- 09:24Results: Obtaning Backbone and Side-chain Exposure Information for Protein Formulations
- 10:28Conclusion
Here, we present detailed protocols for solid-state amide hydrogen/deuterium exchange mass spectrometry (ssHDX-MS) and solid-state photolytic labeling mass spectrometry (ssPL-MS) for proteins in solid powders. The methods provide high-resolution information on protein conformation and interactions in the amorphous solid-state, which may be useful in formulation design.
