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对白细胞从流程的分析招聘基质细胞的影响

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Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

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对白细胞从流程的分析招聘基质细胞的影响

DOI:

10.3791/52480-v

•

11:30 min

•

January 07, 2015

•

Hafsa Munir², G. Ed Rainger², Gerard B. Nash², Helen McGettrick³

¹School of Clinical and Experimental Medicine,University of Birmingham, ²College of Medical and Dental Sciences,University of Birmingham, ³School of Immunity and Infection,University of Birmingham

Chapters

00:05Title
01:28Assembling the Flow System
04:44Setting Up Microslides and the Parallel Plate Flow Chamber Incorporating Filters for Flow
06:59Perfusion of Leukocytes Over Endothelial Cells
07:55Recording Neutrophil Capture and Behavior
09:47Results: Evaluation of Neutrophil Recruitment to Endothelial Cells Cultured Alone or with MSC
11:06Conclusion

Summary

自动翻译

发炎的内皮从流动招募白细胞的能力是由间充质基质细胞调节。我们描述两个体外结合原代人细胞，它可以用于从流动评估中性粒细胞募集和检查的作用是间充质基质细胞在调节这个过程中发挥的模型。

Tags

Endothelial Cells Leukocyte Recruitment Neutrophil Adhesion Mesenchymal Stem Cells In Vitro Vascular Model Flow-based Adhesion Assay TNF-alpha Inflammatory Regulation

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