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عزل وحقن في الوريد من الفئران نخاع العظام المستمدة حيدات
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes
DOI:

09:21 min

December 27, 2014

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Chapters

  • 00:00Title
  • 00:09Isolation and Cultivation of Murine Bone Marrow Derived Monocytes
  • 03:30Tail Vein Injection
  • 05:49Representative Results/Outcome
  • 07:49Conclusion

Summary

自动翻译

Here we present a protocol that generates large amounts of murine monocytes from heterogeneous bone marrow for translational applications. In comparison to others, this new method helps reduce the number of sacrificed animals and lowers costs by avoiding expensive methods such as high gradient magnetic cell separation (MACS).

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