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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
JoVE 杂志
生物学
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JoVE 杂志 生物学
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

24,917 Views

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13:47 min

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February 24, 2015

DOI:

10.3791/52246-v

DOI:
10.3791/52246-v

•

13:47 min
February 24, 2015

•

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1Department of Medicine,Weill Cornell Medical College, 2Institute for Computational Biomedicine,Weill Cornell Medical College, 3Department of Physiology and Biophysics,Weill Cornell Medical College, 4Department of Pathology,University of Michigan

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DNA MethylationBisulfite SequencingReduced RepresentationERRBSBase Pair ResolutionQuantitative MethylationGenomic LociRestriction EnzymeNext-generation SequencingBioinformatics Analysis

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Garrett-Bakelman, F. E., Sheridan, C. K., Kacmarczyk, T. J., Ishii, J., Betel, D., Alonso, A., Mason, C. E., Figueroa, M. E., Melnick, A. M. Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution. J. Vis. Exp. (96), e52246, doi:10.3791/52246 (2015).

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