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亚麻花香浸改造（<em>亚麻</em>）生成的转基因后代具有高转化率
JoVE 杂志
生物学
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JoVE 杂志 生物学
Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate
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亚麻花香浸改造（<em>亚麻</em>）生成的转基因后代具有高转化率

28,437 Views

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08:49 min

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December 19, 2014

DOI:

10.3791/52189-v

DOI:
10.3791/52189-v

•

08:49 min
December 19, 2014

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14 Views
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1Department of Biology,Case Western Reserve University

Summary

Automatically generated

在这里，我们提出了一个协议，通过花卉浸利用农杆菌介导的植物转化改造亚麻。该协议是简单的执行，价格便宜，但得到更高的转化率比亚麻改造当前可用的方法。

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Floral-dip TransformationLinum UsitatissimumAgrobacterium-mediated TransformationBinary Vector PlasmidLinum Insertion Sequence (LIS-1)High Transformation RateT1 SeedsDirect PCRPositive Transformants

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Cite this Article

Bastaki, N. K., Cullis, C. A. Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate. J. Vis. Exp. (94), e52189, doi:10.3791/52189 (2014).

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