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Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
JoVE 杂志
生物学
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JoVE 杂志 生物学
Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples

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11:23 min

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December 22, 2014

DOI:

10.3791/52117-v

DOI:
10.3791/52117-v

•

11:23 min
December 22, 2014

•

84 Views
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1Department of Biology,San Diego State University, 2DOE Joint Genome Institute, 3Department of Molecular, Cellular and Developmental Biology,University of Colorado, 4Department of Pediatrics, School of Medicine,University of Colorado

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Using the cystic fibrosis airway as an example, the manuscript presents a comprehensive workflow comprising a combination of metagenomic and metatranscriptomic approaches to characterize the microbial and viral communities in animal-associated samples.

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MetagenomicsMetatranscriptomicsHost-associated MicrobiomeCystic FibrosisSputumDNA ExtractionRNA ExtractionHigh-throughput SequencingMicrobial CommunityViral CommunityTaxonomyFunctional Analysis

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Lim, Y. W., Haynes, M., Furlan, M., Robertson, C. E., Harris, J. K., Rohwer, F. Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples. J. Vis. Exp. (94), e52117, doi:10.3791/52117 (2014).

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