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망막 색소 상피 세포에 탐식 분석 실험에 대한 돼지 또는 소 광 수용체 외부 세그먼트의 대규모 정화
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JoVE 杂志 免疫与感染
Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells
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망막 색소 상피 세포에 탐식 분석 실험에 대한 돼지 또는 소 광 수용체 외부 세그먼트의 대규모 정화

11,697 Views

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10:02 min

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December 12, 2014

DOI:

10.3791/52100-v

DOI:
10.3791/52100-v

•

10:02 min
December 12, 2014

•

11 Views
, , , ,
1INSERM, U968, 2Sorbonne Universités, UPMC Paris 06, UMR_S 968,Institut de la Vision, 3CNRS, UMR_7210, 4Department of Biological Sciences, Center for Cancer, Genetic Diseases and Gene Regulation,Fordham University

Summary

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This article describes the protocol for the purification of photoreceptor outer segment fragments (POS) via ultracentrifugation from porcine/bovine retinae using homogenization and sucrose gradient centrifugation. This protocol allows the preparation of large stocks of POS aliquots, labeled or unlabeled, that can then be stored at -80 °C.

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Cite this Article

Parinot, C., Rieu, Q., Chatagnon, J., Finnemann, S. C., Nandrot, E. F. Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells. J. Vis. Exp. (94), e52100, doi:10.3791/52100 (2014).

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