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猪或牛光感受器外段的细胞吞噬分析对视网膜色素上皮细胞大规模纯化
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JoVE 杂志 免疫与感染
Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells
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猪或牛光感受器外段的细胞吞噬分析对视网膜色素上皮细胞大规模纯化

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10:02 min

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December 12, 2014

DOI:

10.3791/52100-v

DOI:
10.3791/52100-v

•

10:02 min
December 12, 2014

•

11 Views
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1INSERM, U968, 2Sorbonne Universités, UPMC Paris 06, UMR_S 968,Institut de la Vision, 3CNRS, UMR_7210, 4Department of Biological Sciences, Center for Cancer, Genetic Diseases and Gene Regulation,Fordham University

Summary

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This article describes the protocol for the purification of photoreceptor outer segment fragments (POS) via ultracentrifugation from porcine/bovine retinae using homogenization and sucrose gradient centrifugation. This protocol allows the preparation of large stocks of POS aliquots, labeled or unlabeled, that can then be stored at -80 °C.

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Parinot, C., Rieu, Q., Chatagnon, J., Finnemann, S. C., Nandrot, E. F. Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells. J. Vis. Exp. (94), e52100, doi:10.3791/52100 (2014).

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