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Stab Wound Injury of the Zebrafish Adult Telencephalon: A Method to Investigate Vertebrate Brain Neurogenesis and Regeneration
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Stab Wound Injury of the Zebrafish Adult Telencephalon: A Method to Investigate Vertebrate Brain Neurogenesis and Regeneration

Stab Wound Injury of the Zebrafish Adult Telencephalon: A Method to Investigate Vertebrate Brain Neurogenesis and Regeneration

DOI:
10.3791/51753-v

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09:16 min

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August 04, 2014

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, , ,
1Institute of Toxicology and Genetics,Karlsruhe Institute of Technology

Chapters

  • 00:05Title
  • 01:32Generating a Stab Wound in the Telencephalon
  • 02:21Analyzing the Effect of Telencephalic Injury
  • 04:36Trimming the Agarose Block and Sectioning the Brain
  • 06:36Immunohistochemistry on Vibratome Sections
  • 07:28Telencephalon Stab Wound Results
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

To shed light on the cellular and molecular mechanisms of zebrafish adult neurogenesis and regeneration, we developed a protocol for invasive surgery causing mechanical injuries in the zebrafish adult telencephalon and subsequent monitoring of changes in the stabbed hemisphere by immunohistochemistry or in situ hybridization.

Tags

Keywords: ZebrafishTelencephalonBrainNeurogenesisRegenerationStab WoundInjuryCentral Nervous SystemImmunohistochemistryIn Situ HybridizationCell ProliferationGliogenesisRNA Sequencing

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