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A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide
JoVE 杂志
生物学
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JoVE 杂志 生物学
A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide

A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide

DOI:
10.3791/51710-v

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11:02 min

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October 18, 2014

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, , ,
1Department of Microbiology and Immunology and Department of Pathology,Queen’s University, 2Ask Science Products Inc.

Chapters

  • 00:00Title
  • 01:50Cell Plating and Electroporation
  • 05:38Fixing the Cells
  • 08:55Electroporation of a Peptide to Inhibit Erk Activation
  • 10:11Conclusion

Summary

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This presentation demonstrates a method whereby electroporation of adherent, cultured cells is used for the study of intercellular, junctional communication, while the cells grow on a slide coated with conductive and transparent indium-tin oxide.

Tags

Keywords: Functional AssayGap Junctional CommunicationElectroporationAdherent CellsIndium-tin OxideLucifer YellowCell Membrane PoresCell-to-cell Dye TransferQuantitationSignal Inhibition

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