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Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
 

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics

Article DOI: 10.3791/51656-v • 12:53 min • July 6th, 2014
July 6th, 2014 •
1, 1
1Division of Virology, Department of Pathology, University of Cambridge

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SILAC immunoprecipitation experiments represent a powerful means for discovering novel protein:protein interactions. By allowing the accurate relative quantification of protein abundance in both control and test samples, true interactions may be easily distinguished from experimental contaminants, and low affinity interactions preserved through use of less-stringent buffer conditions.

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Protein Interaction Partners Mammalian Cells SILAC-immunoprecipitation Quantitative Proteomics Immuno-affinity Purification SILAC Immunoprecipitation Protein:protein Interactions Relative Quantification Experimental Contaminants Low Affinity Interactions Buffer Conditions Labeling Of Tissue Culture Cells Stable Isotope Labeled Amino Acids Transfection Immunoprecipitation Affinity Tagged Protein Mass Spectrometry Facility Data Analysis Identification Of Cellular Partners Eukaryotic Translation Initiation Factors
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