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Feeder-fri Udledning af neurale Crest stamceller fra menneskelige pluripotente stamceller
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神经科学
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JoVE 杂志 神经科学
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Feeder-fri Udledning af neurale Crest stamceller fra menneskelige pluripotente stamceller

DOI:
10.3791/51609-v

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10:33 min

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May 22, 2014

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, , ,
1Developmental Biology, Center for Stem Cell Biology,Sloan-Kettering Institute for Cancer Research, 2St. Giles Laboratory of Human Genetics of Infectious Diseases,The Rockefeller University

Chapters

  • 00:05Title
  • 01:38Plating of Human Pluripotent Stem Cells for Differentiation
  • 03:08Induction of Differentiation of Neural Crest Cells
  • 04:54Replating of Neural Crest Cells for Specification
  • 07:37Replating of FACS Sorted Cells
  • 08:56Results: Derivation of Neural Crest Cells from hPSCs using a Feeder Free Protocol
  • 10:09Conclusion

Summary

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自动翻译

Neuralkam (NC) celler afledt af humane pluripotente stamceller (hPSC) har et stort potentiale til at modellere menneskelig udvikling og sygdom og for celleerstatningsterapier. Her et feeder-fri tilpasning af dag i vid udstrækning<em> In vitro</em> Differentiering protokol for afledning af NC celler fra hPSCs præsenteres.

Tags

Feeder-freeNeural Crest Progenitor CellsHuman Pluripotent Stem CellsIn Vitro DifferentiationEmbryonic DevelopmentPeripheral Nervous SystemMelanocytesMesenchymal CellsRegenerative MedicineDisease Modeling

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