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从人多能神经嵴前体细胞的无饲养推导干细胞
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
DOI:

10:33 min

May 22, 2014

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Chapters

  • 00:05Title
  • 01:38Plating of Human Pluripotent Stem Cells for Differentiation
  • 03:08Induction of Differentiation of Neural Crest Cells
  • 04:54Replating of Neural Crest Cells for Specification
  • 07:37Replating of FACS Sorted Cells
  • 08:56Results: Derivation of Neural Crest Cells from hPSCs using a Feeder Free Protocol
  • 10:09Conclusion

Summary

自动翻译

从人类多能干细胞(HPSC)源性神经嵴(NC)细胞具有模拟人类发展和疾病的细胞替代疗法的巨大潜力。这里,一个无饲养适应的目前广泛使用的<em>在体外</em>分化方案用于从hPSCs NC单元的推导给出。

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