Journal
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Capire in anticipo Organogenesis Utilizzando una semplificata<em> In Situ</em> Protocollo di ibridazione in<em> Xenopus</em
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus
DOI:

14:50 min

January 12, 2015

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Chapters

  • 00:05Title
  • 01:52Embryo Preparation
  • 03:45Probe Preparation
  • 06:49In Situ Hybridization
  • 10:49Imaging Embryos
  • 12:38Results: A Simplified In Situ Hybridization Protocols Identifies Specific Organs in Xenopus Embryos
  • 14:26Conclusion

Summary

自动翻译

The Xenopus laevis embryo continues to be exceptionally useful in the study of early development due to its large size and ease of manipulation. A simplified protocol for whole mount in situ hybridization protocol is provided that can be used in the identification of specific organs in this model system.

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