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Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus
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14:50 min
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January 12, 2015
DOI:
1Developmental and Stem Cell Biology,Hospital for Sick Children, 2Children’s Health Research Institute,University of Western Ontario, 3Department of Physiology and Pharmacology,University of Western Ontario, 4Neurosciences and Mental Health,Hospital for Sick Children, 5Department of Paediatrics,University of Western Ontario
Summary
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The Xenopus laevis embryo continues to be exceptionally useful in the study of early development due to its large size and ease of manipulation. A simplified protocol for whole mount in situ hybridization protocol is provided that can be used in the identification of specific organs in this model system.
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Deimling, S. J., Halabi, R. R., Grover, S. A., Wang, J. H., Drysdale, T. A. Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus. J. Vis. Exp. (95), e51526, doi:10.3791/51526 (2015).
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