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Compleet ruggenmergletsel en Brain Dissection Protocol voor Latere Wholemount<em> In Situ</em> Hybridisatie in Larvale Overzeese Lamprei
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Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey
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Compleet ruggenmergletsel en Brain Dissection Protocol voor Latere Wholemount<em> In Situ</em> Hybridisatie in Larvale Overzeese Lamprei

9,758 Views

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08:11 min

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October 14, 2014

DOI:

10.3791/51494-v

DOI:
10.3791/51494-v

•

08:11 min
October 14, 2014

•

5 Views
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1Centre for Neuroregeneration, School of Biomedical Sciences,University of Edinburgh, 2Shriners Hospitals Pediatric Research Center (Center for Neural Repair and Rehabilitation),Temple University School of Medicine, 3Department of Neurology,Temple University School of Medicine

Summary

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Prikken herstellen motoriek na een complete dwarslaesie. Sommige spinale-projecterende neuronen goed regeneratoren en andere niet. Dit document illustreert de technieken voor huisvesting zeeprik larven (en onlangs omgebouwd volwassenen), het produceren van volledige ruggenmerg doorsnijdingen en het voorbereiden wholemount hersenen en ruggenmerg voor in situ hybridisatie.

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Cite this Article

Barreiro-Iglesias, A., Zhang, G., Selzer, M. E., Shifman, M. I. Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey. J. Vis. Exp. (92), e51494, doi:10.3791/51494 (2014).

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