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Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording In Vivo
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神经科学
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JoVE 杂志 神经科学
Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording In Vivo

Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording In Vivo

DOI:
10.3791/51473-v

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09:48 min

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March 29, 2014

•

,
1Hans Berger Department of Neurology,Jena University Hospital, Jena, Germany, 2Integrated Research and Treatment Center, Center for Sepsis Control and Care (CSCC),Jena University Hospital, Jena, Germany

Chapters

  • 00:00Title/Introduction
  • 02:23Preparations for Experiment
  • 03:19Animal Preparation for Recording Procedure
  • 06:00Separating Dorsal Roots and Electrode Positioning
  • 07:35Example Data
  • 08:35Conclusion

Summary

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GABAergic presynaptic inhibition is a powerful inhibitory mechanism in the spinal cord important for motor and sensory signal integration in spinal cord networks. Underlying primary afferent depolarization can be measured by recording of dorsal root potentials (DRP). Here we demonstrate a method of in vivo recording of DRP in mice.

Tags

Keywords: Presynaptic InhibitionDorsal Root PotentialPrimary Afferent DepolarizationGABAergicSpinal CordIn VivoMicePainMotor HyperexcitabilitySuction ElectrodeTransgenic Mouse ModelsSpinal Pathophysiology

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