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薄层色谱(TLC)分离和植物提取物的生物测定识别抗菌化合物
JoVE 杂志
化学
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JoVE 杂志 化学
Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
DOI:

12:04 min

March 27, 2014

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Chapters

  • 00:05Title
  • 01:22Preparation of Thin-layer Plates
  • 03:39Preparation of Developing Chambers for Extract Separation
  • 04:23Loading and Development of TLC Plates
  • 06:04Preparation of Plates for Bioassay
  • 07:41Bacterial Culture and Assay
  • 09:14Visualization of Bioassayed Agar Plates
  • 10:07TLC and Contact Bioautography Assay Results
  • 11:21Conclusion

Summary

自动翻译

方法描述为薄层色谱(TLC)分离植物提取物和生物自显影接触,以确定抗菌代谢产物。该方法适用于红三叶草酚类化合物抑制超氨生产菌(HAB),原产于牛瘤胃的筛选。

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