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Journal / Neuroscience / Mouse Hindbrain Ex Vivo Culture to Study Facial…
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Neuroscience

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JoVE Journal Neuroscience
Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration
 

Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration

Article DOI: 10.3791/51397-v • 10:57 min • March 18th, 2014
March 18th, 2014 •
1, 1,2, 1
1UCL Institute of Ophthalmology, University College London, 2Department of Human Immunology, Centre for Cancer Biology, South Australia

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Embryonic neurons are born in the ventricular zone of the neural tube, but migrate to reach appropriate targets. Facial branchiomotor (FBM) neurons are a useful model to study neuronal migration. This protocol describes the wholemount ex vivo culture of mouse embryo hindbrains to investigate mechanisms that regulate FBM migration.

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Mouse Hindbrain Ex Vivo Culture Facial Branchiomotor Neuron Migration Embryonic Neurons Molecular Signals Neuronal Migration Ventricular Zone Brain Neural Networks Postnatal Life Facial Nuclei Rhombomere 4 Hindbrain Signaling Pathways FBM Migration E11.5 Mouse Embryos Open Book Preparation Cell Culture Inserts Function-blocking Antibodies Small Molecules Culture Media Heparin Beads Recombinant Proteins
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