JoVE Journal
Neuroscience
Neuroscience
需要订阅 JoVE 才能查看此 内容. 登录或开始免费试用。
章节
总结概括
Here we demonstrate the use of fluorescent Alexa dye coupled to α-bungarotoxin to measure GABA A receptor surface localization and endocytosis in hippocampal neurons. Through the use of constructs bearing a short extracellular tag that binds α-bungarotoxin, analysis of plasma membrane protein endocytic trafficking can be achieved.
