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Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
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Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

12,097 Views

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08:57 min

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March 10, 2014

DOI:

10.3791/51346-v

DOI:
10.3791/51346-v

•

08:57 min
March 10, 2014

•

2 Views
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1Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine,Marshall University

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Here we describe a protocol using the mini-himar1 mariner transposon-mediated mutagenesis for generating a high-density insertion mutant library to screen, isolate and identify novel alginate regulators in the prototypic Pseudomonas aeruginosa strain PAO1.

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Withers, T. R., Yin, Y., Yu, H. D. Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis. J. Vis. Exp. (85), e51346, doi:10.3791/51346 (2014).

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