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Journal / Immunology and Infection / Detection of Fluorescent Nanoparticle…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
 

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

Article DOI: 10.3791/51345-v • 07:31 min • March 28th, 2014
March 28th, 2014 •
1, 1,2, 3, 3, 3, 1, 1
1Center for Micro-BioRobotics @SSSA, Istituto Italiano di Tecnologia, 2Department of Biology, University of Pisa, 3Center for Biomolecular Nanotechnologies @UniLe, Istituto Italiano di Tecnologia

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Automatic Translation

Analysis of nanoparticle interaction with defined subpopulations of immune cells by flow cytometry.

Tags

Fluorescent Nanoparticles Primary Immune Cell Subpopulations Flow Cytometry Nanoparticle Interaction Therapeutic Purposes Diagnostic Purposes Fast And Reliable Method Antibody-mediated Magnetic Isolation Forward-scattered Light (FSC) Side-scattered Light (SSC) Fluorescently Labeled Antibodies Cell Surface Receptors External Stimuli Physiological State Morphological State 50 Nm FITC-SiO2 Nanoparticles Nanostructured Materials Human Blood Immune Cells Cell Fluorescence Side-scattered Light Increase Incubation With Nanoparticles Time Dependence Concentration Dependence Rhodamine-SiO2 Nanoparticles Primary Microglia Central Nervous System Resident Immune Cells
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