Journal
/
生物学
/
Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

14,202 Views

•

09:22 min

•

November 26, 2013

DOI:

10.3791/51153-v

DOI:
10.3791/51153-v

•

09:22 min
November 26, 2013

•

12 Views
, , ,
1The Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Department of Molecular Biology,Princeton University, 3Division of Chemistry and Chemical Engineering,California Institute of Technology

Summary

Automatically generated

This protocol describes an experimental procedure for the rapid construction of artificial transcription factors (ATFs) with cognate GFP reporters and quantification of the ATFs ability to stimulate GFP expression via flow cytometry.

Explore More Videos

Synthetic BiologyTranscription FactorsGene ExpressionReporter SystemsGFPHomologous RecombinationArtificial Transcription FactorsConditional Gene Expression

Read Article

Cite this Article

McIsaac, R. S., Oakes, B. L., Botstein, D., Noyes, M. B. Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters. J. Vis. Exp. (81), e51153, doi:10.3791/51153 (2013).

Download .ris file

Copy

Share Video

.

Copy