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Isolation and Culture of Endothelial Cells from the Embryonic Forebrain
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神经科学
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JoVE 杂志 神经科学
Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

Isolation and Culture of Endothelial Cells from the Embryonic Forebrain

DOI:
10.3791/51021-v

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12:02 min

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January 23, 2014

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,
1Department of Psychiatry,Harvard Medical School, 2Angiogenesis and Brain Development Laboratory, Division of Basic Neuroscience,McLean Hospital

Chapters

  • 00:05Title
  • 01:45Removal of Embryos and Dissection of Telencephalon
  • 02:53Cell Isolation
  • 04:43Magnetic Labeling and Separation
  • 07:26Cultured Periventricular Endothelial Cell Characterization
  • 11:24Conclusion

Summary

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This video demonstrates an easy and reliable strategy for preparation of pure cultures of endothelial cells from the embryonic forebrain within 10-12 days and will be useful for research focused on many aspects of cerebral angiogenesis.

Tags

Embryonic ForebrainEndothelial CellsPial Vascular NetworkPeriventricular Vascular NetworkIsolationCulturePurificationCD31/PECAM-1AngiogenesisNeurovascular Development

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