JoVE Journal
Immunology and Infection
Immunology and Infection
需要订阅 JoVE 才能查看此 内容. 登录或开始免费试用。
章节
总结概括
One key to successful investigation of microglial biology is the preservation of microglial immunofunction ex vivo during isolation from CNS tissue. Isolating microglia via rotary shaking results in highly pure and immunofunctional cell cultures as assessed by fluorescent imaging, immunocytochemistry, and ELISA following microglia activation with the proinflammatory stimuli lipopolysaccharide (LPS) and Pam3CSK4 (Pam).