需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
15,453 Views
•
09:12 min
•
January 30, 2014
DOI:
Summary
Automatically generated
One key to successful investigation of microglial biology is the preservation of microglial immunofunction ex vivo during isolation from CNS tissue. Isolating microglia via rotary shaking results in highly pure and immunofunctional cell cultures as assessed by fluorescent imaging, immunocytochemistry, and ELISA following microglia activation with the proinflammatory stimuli lipopolysaccharide (LPS) and Pam3CSK4 (Pam).
Read Article
Cite this Article
Daniele, S. G., Edwards, A. A., Maguire-Zeiss, K. A. Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates. J. Vis. Exp. (83), e51005, doi:10.3791/51005 (2014).
Copy