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Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)
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JoVE 杂志 生物学
Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)

Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)

DOI:
10.3791/50968-v

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10:36 min

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December 05, 2013

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, , ,
1Neuroscience Center of Excellence,Louisiana State University Health Sciences Center

Chapters

  • 00:05Title
  • 01:39Prepare Samples for Tandem Affinity Purification Procedure
  • 04:02Tandem Affinity Purification Procedure
  • 07:44Results: Identification of Interacting Proteins in the Drosophila Brain by Tandem Affinity Purification
  • 09:31Conclusion

Summary

自动翻译

自动翻译

Drosophila is famous for its powerful genetic manipulation, but not for its suitability of in-depth biochemical analysis. Here we present a TAP-based procedure to identify interacting partners of any protein of interest from the fly brain. This procedure can potentially lead to new avenues of research.

Tags

Keywords: Protein-protein InteractionDrosophilaAdult HeadsTandem Affinity Purification (TAP)Mass SpectrometryGAL4/UAS SystemProtein ComplexGenetic ScreensBiochemical ScreensNeurobiology

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