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Imaging Centrosomes in Fly Testes
JoVE 杂志
生物学
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JoVE 杂志 生物学
Imaging Centrosomes in Fly Testes

Imaging Centrosomes in Fly Testes

DOI:
10.3791/50938-v

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09:41 min

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September 20, 2013

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, , ,
1Department of Biological Sciences,University of Toledo

Chapters

  • 00:05Title
  • 01:14Preparation of Live Testes
  • 02:26Preparation of Fixed Testes
  • 03:27Preparation of Immunostained Testes
  • 07:59Analyzing Chromosome Elongation
  • 09:18Conclusion

Summary

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自动翻译

Imaging of centrosomal proteins during Drosophila spermatogenesis is a powerful method to identify new proteins critical for centrosome biology as well as to elucidate the particular function of known players in this process.

Tags

Keywords: CentrosomesMicrotubulesCell CycleCell DifferentiationCell DivisionCiliaDrosophilaSpermatogenesisGenetic ModelMutantsLight MicroscopyGenetically-tagged MarkersGenetic Screens

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