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Single-plant, Sterile Microcosms for Nodulation and Growth of the Legume Plant Medicago truncatula with the Rhizobial Symbiont Sinorhizobium meliloti
Chapters
- 00:05Title
- 02:37Preparation of M. truncatula A17 Seedlings: Sterilization of Seeds
- 05:01Preparation of M. truncatula A17 Seedlings: Germination
- 07:00Preparation of Individual Plate Microcosms
- 08:29Laying out M. truncatula Seedlings on the Individual Microcosms
- 09:59Inoculation and Sealing of Microcosms
- 12:10Examination of Root Systems and Quantitation of Symbiotic Phenotypes
- 13:02Results: Symbiotic Growth of M. truncatula and S. meliloti in Sterile, Single-plant Microcosms
- 19:03Conclusion
Growth of Medicago truncatula plants in symbiosis with the nitrogen-fixing bacteria Sinorhizobium meliloti in individual, sterile microcosms made from standard laboratory plates permits frequent examination of root systems and nodules without compromising sterility. Plants can be maintained in these growth chambers for up to 9 weeks.
