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Chapters
- 00:05Title
- 01:30Postnatal Electroporation
- 03:36Preparation of Acute Brain Slice Cultures
- 05:41Time-lapse Imaging of Neuroblast Migration
- 06:57Analyzing Neuroblast Migration
- 08:51Results: Time-lapse Imaging of Migrating Neuroblasts in Mouse Acute Brain Slices
- 10:13Conclusion
Neuroblast migration is a fundamental event in postnatal neurogenesis. We describe a protocol for efficient labeling of neuroblasts by in vivo postnatal electroporation and subsequent visualization of their migration using time-lapse imaging of acute brain slices. We include a description for the quantitative analysis of neuroblast dynamics by video tracking.
