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Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals
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神经科学
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JoVE 杂志 神经科学
Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals

Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals

DOI:
10.3791/50884-v

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09:53 min

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February 11, 2014

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, , ,
1CGphiMC, UMR CNRS 5534,University of Lyon 1

Chapters

  • 00:05Title
  • 01:45Spinal Cord Dissection
  • 05:03FP Culture and FP Conditioned Medium Harvesting
  • 06:35Commissural Neuron Dissection and Treatments
  • 08:15Results: Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals
  • 08:58Conclusion

Summary

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The floor plate is a crucial structure of the developing spinal cord, providing diffusible signals to progenitors and differentiating neurons. We describe a method to produce floor-plate conditioned medium, to apply it to fresh spinal cord tissue, and to assess the consequences on proteins of interest by biochemistry.

Tags

Keywords: Floor PlateConditioned MediumSpinal Cord DevelopmentAxon GuidanceMorphogensCommissural AxonsGuidance CuesSignaling Pathways

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