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Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

DOI:
10.3791/50883-v

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10:30 min

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January 12, 2014

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, , ,
1University Claude Bernard Lyon1, CGphiMC UMR CNRS 5534,University of Lyon

Chapters

  • 00:05Title
  • 02:05Transfecting COS7 Cells
  • 05:26In ovo Electroporation of pHluorin-PlexinA1 Construct
  • 08:20Expression of pHluorin-PlexinA1
  • 09:26Conclusion

Summary

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We describe here the use of a pH-sensitive green fluorescent protein variant, pHluorin, to study the spatio-temporal dynamics of axon guidance receptors trafficking at the cell surface. The pHluorin-tagged receptor is expressed both in cell culture and in vivo, using electroporation of the chick embryo.

Tags

PHluorinAxon Guidance ReceptorsCell CultureChick EmbryoSpatio-temporal DynamicsCell Surface DynamicsGrowth ConesSignaling MechanismsPH-dependent FluorescenceGFP VariantPlasma MembraneIn VitroIn VivoSpinal Cord

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